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H. M. Suresh , B. C. Hatapakki , S. I. Shivakumar , C. S. Hallikeri , B. M. Swamy , V. K. Chandur

Abstract

Objective: To evaluate ethanol extract of pods of Cassia auriculata Linn at different doses (100, 200 mg / kg, p.o.) for laxative activity in rats. Materials and Methods: The ethanol extract of pods of Cassia auriculata was obtained by continuous soxhlet extraction and further assessed for laxative activity by charcoal meal test and feacal output in experimental rats and compared with standard senna. The extract was subjected to qualitative chemical analysis to identify the phytoconstituents. Results: Upon evaluation of Laxative activity on experimental animals, the ethanol extract at the dose 200 mg / kg, p.o. showed significant (P<0.001) laxative activity as observed from different evaluation parameters. However, the same at the dose 100 mg / kg, p.o. was less significant. The ethanol extract showed the presence of flavonoids, triterenoids, tannins, sterols and anthracene derivatives. Conclusion: From the results, it is revealed that, the active ethanol extract (200 mg / kg, p.o.) of Cassia auriculata pods is worthwhile to develop the bioactive principle for laxative activity and it is also concluded that, the anthracene derivatives present, could be attributed for the laxative activity.

Keywords

Cassia auriculata, Pods, Laxative, Anthracene Derivatives, Ethanol Extract

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H. M. Suresh , B. C. Hatapakki , S. I. Shivakumar , C. S. Hallikeri , B. M. Swamy , V. K. Chandur

Abstract

Objective: To evaluate ethanol extract of pods of Cassia auriculata Linn at different doses (100, 200 mg / kg, p.o.) for laxative activity in rats. Materials and Methods: The ethanol extract of pods of Cassia auriculata was obtained by continuous soxhlet extraction and further assessed for laxative activity by charcoal meal test and feacal output in experimental rats and compared with standard senna. The extract was subjected to qualitative chemical analysis to identify the phytoconstituents. Results: Upon evaluation of Laxative activity on experimental animals, the ethanol extract at the dose 200 mg / kg, p.o. showed significant (P<0.001) laxative activity as observed from different evaluation parameters. However, the same at the dose 100 mg / kg, p.o. was less significant. The ethanol extract showed the presence of flavonoids, triterenoids, tannins, sterols and anthracene derivatives. Conclusion: From the results, it is revealed that, the active ethanol extract (200 mg / kg, p.o.) of Cassia auriculata pods is worthwhile to develop the bioactive principle for laxative activity and it is also concluded that, the anthracene derivatives present, could be attributed for the laxative activity.

Keywords

Cassia auriculata, Pods, Laxative, Anthracene Derivatives, Ethanol Extract

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S. I. Shivakumar , H. M. Suresh , C. S. Hallikeri , B. C. Hatapakki , J. S. Handiganur , Kuber Sankh , B. Shivakumar

Abstract

Objective: To evaluate the anticonvulsant effect of Cyperus rotundus Linn rhizomes against maximal electroshock (MES) and pentylenetetrazole (PTZ) induced tonic seizers in albino rats. Materials and methods: Ethanol extract of Cyperus rotundus Linn rhizomes were obtained by continuous hot soxhlet extraction. The extract was assessed for anticonvulsant activity by measuring (sec) the duration of tonic flexion, tonic extensor, clonus, stupor and recovery phase in rats. Results: Phytochemical investigation of ethanol extract revealed the presence of flavonoids, vitamins and carbohydrates. The ethanol extract (100 mg / kg, p.o.) reduced hind limb extension and duration of convulsion significantly, (P < 0.001) which was comparable to standard drug phenytoin (25 mg / kg, i.p.) and diazepam (4 mg /kg, i.p.) respectively. Conclusion: The ethanol extract of Cyperus rotundus rhizomes is worthwhile to develop the potent phytoconstituent for treatment of epilepsy and the flavonoids present in ethanol extract could be attributed for anticonvulsant activity.

Keywords

Anticonvulsant, Cyperus Rotundus, Rhizomes, Maximal Electroshock, Pentylenetetrazole

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S. I. Shivakumar ¹, Amreen Begum ¹, Syeda Sana ¹, Ansari Firdous ¹

Affiliations
1 Department of Pharmacology, HKE’S Matoshree Taradevi Rampure Institute of Pharmaceutical Sciences Gulbarga, Sedam Road, Gulbarga-585105, Karnataka, IN

Abstract

Due to the development of antibiotic resistance and the outbreak of infectious diseases caused by resistant pathogenic bacteria, the pharmaceutical companies and the researchers are now searching for new unconventional antibacterial agents. Hence, there is an ever-growing need to develop new antimicrobial compounds. In the present study various medicinal plants were selected to check the antimicrobial activity and Cyperus rotundus (linn) was found to have the best activity against microbes. Pet ether extract, chloroform extract and methanol extract were screened for their antimicrobial activity against various microbes which are responsible for inducing diarrhoea viz. Staphylococcus aureus, Bacillus cereus, Escherichia coli and Campylobacter jejunir using agar well diffusion method. Methanolic extract at the dose of 100 μg, have shown good antibacterial activity where as Methanolic extract at the dose of 80μg have shown moderate activity, using Loperamide as a standarad drug, against Staphylococcus aureus, Bacillus cereus, Escherichia coli and Campylobacter jejuni with zones of inhibition ranging from 19 mm to 24 mm. It might be concluded from the present study that the potential of this herb to produce useful antimicrobial compounds is great and must be better explored

Keywords

Cyperus rotundus (linn), Pet Ether, Chloroform and Methanolic Extract Loperamide Antimicrobial Activity

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